Tanvi Javkar
Slow cycling intestinal stem cell and Paneth cell responses to Trichinella spiralis infection
Javkar, Tanvi; Hughes, Kevin R.; Sablitzky, Fred; Behnke, Jerzy M.; Mahida, Yashwant R.
Authors
Kevin R. Hughes
Fred Sablitzky
Jerzy M. Behnke
YASH MAHIDA yash.mahida@nottingham.ac.uk
Professor of Medicine
Abstract
There is limited information regarding responses by slow cycling stem cells during T. spiralis-induced T-cell mediated intestinal inflammation and how such responses may relate to those of Paneth cells. Transgenic mice, in which doxycycline induces expression of histone 2B (H2B)-green fluorescent protein (GFP), were used. Following discontinuation of doxycycline (“chase” period), retention of H2B-GFP enabled the identification of slow cycling stem cells and long-lived Paneth cells. Inflammation in the small intestine (SI) was induced by oral administration of T. spiralis muscle larvae. Epithelial retention of H2B-GFP per crypt cell position (cp) was studied following immunohistochemistry and using the Score and Wincrypts program. Compared to non-infected controls, there was significant reduction in the number of H2B-GFP-retaining stem cells in T. spiralis-infected small intestines. H2B-GFP-retaining stem cells peaked at around cp 4 in control sections, but smaller peaks at higher cell positions (>10) were seen in sections of inflamed small intestines. In the latter, there was a significant increase in the total number of Paneth cells, with significant reduction in H2B-GFP-retaining Paneth cells, but a marked increase in unlabelled (H2B-GFP-negative) Paneth cells. In conclusion, following T. spiralis-infection, putative slow cycling stem cell numbers were reduced. A marked increase in newly generated Paneth cells at the crypt base led to higher cell positions of the remaining slow cycling stem cells.
Citation
Javkar, T., Hughes, K. R., Sablitzky, F., Behnke, J. M., & Mahida, Y. R. (2020). Slow cycling intestinal stem cell and Paneth cell responses to Trichinella spiralis infection. Parasitology International, 74, Article 101923. https://doi.org/10.1016/j.parint.2019.05.001
Journal Article Type | Article |
---|---|
Acceptance Date | May 1, 2019 |
Online Publication Date | May 2, 2019 |
Publication Date | 2020-02 |
Deposit Date | May 10, 2019 |
Publicly Available Date | May 3, 2020 |
Journal | Parasitology International |
Print ISSN | 1383-5769 |
Electronic ISSN | 1873-0329 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 74 |
Article Number | 101923 |
DOI | https://doi.org/10.1016/j.parint.2019.05.001 |
Keywords | Stem cells; Paneth cells; Trichinella spiralis |
Public URL | https://nottingham-repository.worktribe.com/output/2037955 |
Publisher URL | https://www.sciencedirect.com/science/article/pii/S1383576918304215?via%3Dihub |
Contract Date | May 10, 2019 |
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